GENE DELIVERY BY MICRONEEDLE
Microseeding uses repeated injection from solid microneedles to deliver genetic material directly into an external or internal target. Our primary targets are skin cells and periosteal cells. Other tissue and organs, such as muscle, heart, stomach, and bowel are also treatable. Microseeding can be used with naked DNA or in combination with any viral or chemical vector.
Microseeding has many advantages:
- -Effective, efficient, versatile
-Works with DNA plasmid or vector Potentiates most vectors
-High level of safety, predictability, and reproducibility
-Economical, practical, and customizable to the patient
Combined with the use of the Wound Chamber system this also allows for non-invasive assessment of the success of the transfer by assaying for the presence of the expressed protein in wound fluid, in contrast to the prior art use of invasive techniques, such as biopsies, in order to achieve the same assessment of early expression.
Cells cultured in the chamber can be genetically modified. The genetic engineering can be done in the laboratory, or directly in the patient. If the cells were not covered by the wound chamber, most of them would desiccate and die. The chamber seals the wound from the outside, eliminating the spread of genetic material and vectors to places outside the wound. At the same time, the chamber prevents the accidental introduction of undesired contamination into the wound.